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Moss Methods

 

The Freiburg standard methods for Physcomitrella patens cell culture, protoplastation and transformation are described here (all links as PDF Document):

Introduction

Cell culture

Sporophyte induction

Transformation of protoplasts

Targeted knockouts

Analysis of transformants

 

If you want to cite the methods or want to have a look at the original publications:

Hohe A., R. Reski (2002): Optimisation of a bioreactor culture of the moss Physcomitrella patens for mass production of protoplasts. Plant Sci. 163, 69-74.

Hohe, A., S.A. Rensing, M. Mildner, D. Lang, R. Reski (2002): Day length and temperature strongly influence sexual reproduction and expression of a novel MADS-box gene in the moss Physcomitrella patens. Plant Biol. 4, 595-602.

Schween G., S. Fleig, R. Reski (2002): High-throughput-PCR screen of 15,000 transgenic Physcomitrella plants. Plant Mol. Biol. Rep. 20, 43-47.

Hohe, A., T. Egener, J. Lucht, H. Holtorf, C. Reinhard, G. Schween, R. Reski (2004): An improved and highly standardised transformation procedure allows efficient production of single and multiple targeted gene knockouts in a moss, Physcomitrella patens. Current Genet. 44, 339-347.

Schulte, J., R. Reski (2004): High-throughput cryopreservation of 140000 Physcomitrella patens mutants. Plant Biol. 6, 119-127.

Heintz, D., V. Wurtz, A. High, A. Van Dorsselaer, R. Reski, E. Sarnighausen (2004): An efficient protocol for the identification of protein phosphorylation in a seedless plant, sensitive enough to detect members of signalling cascades. Electrophoresis 25, 1149-1159.

 

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